No sexual confusion for chicken cells
Wednesday, 03.24.2010, 12:43pm (GMT+3)
One side of the gynandromorph chicken has the brown
feathers, small wattle, and smaller breast of a hen, whereas the other
side boasts the white feathers, large wattle, and
muscular breast of a rooster. The cluster of seemingly nondescript cells
that grows into a chick has a stronger sense of
identity than you'd think. Rather than waiting for hormonal cues from
the
sex organs, the cells know whether they're male or
female from the start, a new study reveals. The discovery challenges the
standard picture of how sexual differences develop in
vertebrates. In humans, somatic cells—the generic cells that grow into
muscle, bone, and organs—start off unisex. Even though
chromosomes mark the cells as male or female, they don't head down
that path until after the gonads—testes in a male,
ovaries in a female—begin developing and secreting hormones at about 7
weeks. Sex is decided at this point by a key gene on
the male-specific Y chromosome, which signals the embryo to develop
testes.
Hormones then drive the erstwhile unisex cells to
develop male or female features.
For the past 50 years, biologists assumed that all
vertebrates needed these cues before their cells took on a male or
female
identity. So Michael Clinton, a developmental
biologist at the University of Edinburgh in the United Kingdom, couldn't
understand
why it was so difficult to find the key
sex-determining gene in chicken chromosomes. Then he got a call from an
industry acquaintance
who on his visits to poultry breeders occasionally
noticed strange birds that were half brown and half white and had an
oddly
lopsided breast. Clinton agreed to take a look. The
chickens were gynandromorphs, with their sex split down the middle. They
appeared male on one side and female on the other.
Unlike humans, where women have two X chromosomes and men have an X and
a Y, male chickens normally have two Z chromosomes,
whereas females have a Z and a W chromosome. Clinton’s team sampled
blood
and skin from both sides of each chicken, expecting to
find that cells from one side or the other lacked a sex chromosome.
This might give them a clue as to which chromosome,
the Z or the W, held the key gene for determining sex-specific traits,
Clinton thought.
"It turned out that was completely rubbish," Clinton
says. Both sides of the chickens included normal male (ZZ) and female
(ZW) cells. But male cells dominated one side, whereas
female cells dominated the other, accounting for the split appearance.
The same was true of cells taken from the chickens'
breast muscles and wattles. This result suggested that chicken cells,
unlike mammalian cells, had their own sex identity
that was to some extent independent of what hormones the gonads
produced.
Studies of normal chicken embryos bolstered the case.
Just 18 hours after fertilization, long before gonads began to form,
the embryos exhibited either "maleness" or
"femaleness," as indicated by expression of sex-specific RNA molecules.
To test
whether these cells were really male or female, the
team also removed them from early embryos that hadn't formed gonads yet
and delicately placed them in eggs containing male and
female chicks, creating partial gynandromorphs. After a week, some
implanted cells had become part of the gonads of the
opposite-sex host. But they resolutely held on to their sex identities,
the team reports in tomorrow's issue of Nature.
So instead of waiting for a cue from the gonads,
somatic cells in chickens seem to know their own sex. The researchers
speculate
that the cells somehow drive masculine or feminine
development, probably by triggering or repressing the activity of a gene
called DMRT-1 on the Z chromosome. Last year,
researchers found that suppressing this gene in male chick embryos made
them
develop a testis with feminine characteristics.
Although scientists had begun to suspect that the standard mammalian
model
for sex determination wasn't the whole story for all
vertebrates, "this study very clearly shows it for the first time
certainly
in birds," says Craig Smith, a developmental biologist
at the Murdoch Childrens Research Institute in Parkville, Australia,
whose team discovered DMRT-1. The mechanism "might be
more pervasive amongst animals than we've thought."
ScienceNow
|